The objective of the study was to develop a clinical confo- cal microlaparoscope for imaging ovary epithelium in vivo with the long- term objective of diagnosing cancer in vivo. A confocal microlaparoscope was developed and used to image the ovaries of 21 patients in vivo using fluorescein sodium and acridine orange as the fluorescent contrast agents. The device was tested in vivo and demonstrated to be safe and function as designed. Real-time cellular visualization of ovary epithelium was demonstrated. The confocal microlaparoscope represents a new type of in vivo imaging device. With its ability to image cellular details in real time, it has the potential to aid in the early diagnosis of cancer. Initially the device may be used to locate unusual regions for guided biopsies. In the long term, the device may be able to supplant traditional biopsies and allow the surgeon to identify early-stage ovarian cancer. You can read the full journal article. Below is a video from the instrument in use.
This publication represented 1 of 3 journal articles that comprimised my dissertation. My contribution was the design and development of the next generation confocal microlaparascope, software development, lens design, and clinical testing of the device with Kenneth Hatch, Andy Rouse, and Art Gimitro. Both Art (my advisor) and Andy were exceptional mentors and collaborators for this work. I couldn't have done it without them.
Citation: Anthony A Tanbakuchi, Joshua A Udovich, Andrew R Rouse, Kenneth D Hatch, and Arthur F Gmitro. In vivo imaging of ovarian tissue using a novel confocal microlaparoscope. Am J Obstet Gynecol, Oct 2010. PDF